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Post by DAVID user on Jul 22, 2020 10:04:57 GMT -5
Hello DAVID team and users,
I'm inputting a list of 365 genes that I hope to look at enriched GO terms (BP) with. However, I keep getting an error that my list contains more than 3000 genes even though my list has only 365. Is there a reason for this error? How can I avoid it?
Thanks!
From,
Eva
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Post by DAVID user on Jul 22, 2020 10:05:42 GMT -5
Hi Eva,
I suspect that your list contains gene symbols and DAVID is mapping them to every species which leads to a very large list. Do you receive the message on upload or when you try to perform the enrichment analysis?
Regards, Brad
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Post by DAVID user on Jul 22, 2020 10:05:54 GMT -5
Hi Brad,
Thanks for the quick reply. It was only when I tried to perform the enrichment analysis. My gene list is exported out of Seurat, and I believe they are official gene names. What I did was upload this list, then specified mouse in the second tab.
I started fresh today, and it worked! Thanks for your insight.
From,
Eva
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Post by DAVID user on Jul 22, 2020 10:06:04 GMT -5
Hi again Brad,
Actually, now it is not working again when I tried another shorter gene list. In case it had something to do with my gene names, I tried the converter. However, it is stuck at 30%. Is there something about the system where I can't re-process anything until the previous "expires"...?
From,
Eva
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Post by DAVID user on Jul 22, 2020 10:06:16 GMT -5
Hi Eva, Please try the steps in the attached pdf to make sure that you have selected your species of interest for both the uploaded gene list and background. Regards, Brad DAVID_species_selection.pdf (118.73 KB) |
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Post by DAVID user on Jul 22, 2020 10:07:27 GMT -5
Eva,
I wonder if your browser is causing problems for you as well. When I first started using DAVID, I was using Firefox as my browser and never got a sense when jobs were finished, I assume because it was blocking pop-ups. I switched to Google Chrome, and it works fine.
Wes
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